Wu Xuecheng, Gréhan Gérard, Meunier-Guttin-Cluzel Siegfried, Chen Linghong, Cen Kefa
State Key Laboratory of Clean Energy Utilization, Institute for Thermal Power Engineering, Zhejiang University, Hangzhou, China.
Opt Lett. 2009 Mar 15;34(6):857-9. doi: 10.1364/ol.34.000857.
The holographic microscopy technique is a strong contender for dynamic three-dimensional (3D) measurement of small particles (typically smaller than 5 microm) in microchannels. However, there is a big challenge to accurately measure the size of such small particles. The traditional hologram reconstruction method was numerically investigated. It is found that the error level, especially for the size measurement, is higher than expected, even in an ideal situation without consideration of noise. An alternative way based on Lorenz-Mie (LM) calculations was then presented. The intensity distribution of the fringe pattern on the particle hologram is directly used and compared with the ones calculated using an LM-based program. Various cases for particle sizes from 0.5 to 5 microm and recording distances from 5 to 500 microm are tested. The results show that the accuracy in particle sizing can be significantly improved.
全息显微镜技术是对微通道中小颗粒（通常小于5微米）进行动态三维（3D）测量的有力竞争者。然而，准确测量此类小颗粒的尺寸面临着巨大挑战。对传统全息图重建方法进行了数值研究。结果发现，即使在不考虑噪声的理想情况下，误差水平，尤其是尺寸测量的误差水平，也高于预期。随后提出了一种基于洛伦兹 - 米氏（LM）计算的替代方法。直接使用粒子全息图上条纹图案的强度分布，并将其与使用基于LM的程序计算出的强度分布进行比较。测试了粒径从0.5到5微米以及记录距离从5到500微米的各种情况。结果表明，颗粒尺寸测量的精度可以得到显著提高。
