数字共聚焦显微镜。

Digital confocal microscope.

作者信息

Goy Alexandre S, Psaltis Demetri

机构信息

Optics Laboratory, École Polytechnique Fédérale de Lausanne, Station 17, Lausanne 1015, Switzerland.

出版信息

Opt Express. 2012 Sep 24;20(20):22720-7. doi: 10.1364/OE.20.022720.

DOI:10.1364/OE.20.022720

PMID:23037422

Abstract

We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally produce three-dimensional images. Several methods to treat the data are presented. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown.

摘要

我们通过实验演示了一种基于数字全息术的扫描共聚焦显微镜技术。该方法依赖于对扫描点进行数字全息记录。以这种方式收集的数据包含数字生成三维图像所需的所有必要信息。文中介绍了几种处理数据的方法。展示了上皮细胞和小鼠脑组织的反射和透射图像示例。