Kemper Björn, Carl Daniel, Schnekenburger Jürgen, Bredebusch Ilona, Schäfer Marcus, Domschke Wolfram, von Bally Gert
University of Muenster, Laboratory of Biophysics, Germany.
J Biomed Opt. 2006 May-Jun;11(3):34005. doi: 10.1117/1.2204609.
Digital holographic microscopy provides new facilities for contactless and marker-free quantitative phase contrast imaging. In this work, a digital holographic microscopy method for the integral refractive index determination of living single cells in cell culture medium is presented. Further, the obtained refractive index information is applied to full field thickness and shape determination of adherent pancreas tumor cells, as well as for analysis of drug-induced dynamic changes of a single cell's cytoskeleton. The results demonstrate that digital holographic microscopy is a quantitative phase contrast technique for living cells under conventional laboratory conditions.
数字全息显微镜为非接触式和无标记定量相衬成像提供了新的手段。在这项工作中，提出了一种用于测定细胞培养基中活单细胞积分折射率的数字全息显微镜方法。此外，所获得的折射率信息被应用于贴壁胰腺肿瘤细胞的全场厚度和形状测定，以及单细胞细胞骨架药物诱导动态变化的分析。结果表明，数字全息显微镜是一种在传统实验室条件下用于活细胞的定量相衬技术。
