Saeki T, Morioka T, Arakawa M, Shimizu F, Oite T
Department of Medicine (II), Niigata University School of Medicine, Japan.
Am J Pathol. 1991 Oct;139(4):949-57.
The effects of direct cell contact between endothelial (ECs) and mesangial cells (MCs) on MCs proliferation were examined in a coculture system in vitro. Mitomycin C treated ECs (M-ECs) were plated on culture dishes and MCs were cocultured with these M-ECs. Cell number was measured at the end of days 1, 3, and 5. In the coculture system with direct contact, the growth of cocultured MCs was modulated as follows: 1) the growth of MCs was inhibited up to day 3, and 2) a high level of proliferation was observed between days 3 and 5. This biphasic pattern of growth could not be detected in coculture of fibroblasts with MCs. In coculture without direct contact, using intercup chambers, the kinetics in cell proliferation between cocultured MCs and MCs alone were essentially the same. Conditioned media derived from cocultures up to day 3 in a contact-dependent manner inhibited the 3H-thymidine uptake of MCs. From these results, it would thus appear that MCs proliferation is regulated by intercellular contact with ECs.
在体外共培养系统中，研究了内皮细胞（ECs）与系膜细胞（MCs）之间直接细胞接触对MCs增殖的影响。将丝裂霉素C处理过的内皮细胞（M-ECs）接种于培养皿上，然后将系膜细胞与这些M-ECs进行共培养。在第1、3和5天结束时测量细胞数量。在直接接触的共培养系统中，共培养的系膜细胞生长受到如下调节：1）直到第3天，系膜细胞的生长受到抑制；2）在第3天至第5天之间观察到高水平的增殖。在成纤维细胞与系膜细胞的共培养中未检测到这种双相生长模式。在使用分隔小室的非直接接触共培养中，共培养的系膜细胞与单独培养的系膜细胞之间的细胞增殖动力学基本相同。共培养至第3天的条件培养基以接触依赖的方式抑制了系膜细胞对3H-胸腺嘧啶核苷的摄取。从这些结果来看，似乎系膜细胞的增殖是由与内皮细胞的细胞间接触调节的。
