Canning B J, Hmieleski R R, Spannhake E W, Jakab G J
Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
Am J Physiol. 1991 Oct;261(4 Pt 1):L277-82. doi: 10.1152/ajplung.1991.261.4.L277.
Continuous ozone exposure (0.5 ppm, 1-14 days) reduced the phagocytic activity of murine alveolar and peritoneal macrophages. The response of peritoneal macrophages to ozone was virtually indistinguishable from the response of alveolar macrophages. When added exogenously, prostaglandin E2 (PGE2) inhibited alveolar and peritoneal macrophage phagocytosis. To test the hypothesis that prostanoids mediated the effects of ozone on macrophages, PGE levels of bronchoalveolar lavage fluid (BALF) and the phagocytic activity of macrophages from ozone-exposed mice pretreated with cyclooxygenase inhibitors were measured. PGE levels in BALF were increased following ozone exposure, with high levels of PGE associated with large decreases in phagocytic activity. Pretreatment with indomethacin and d-naproxen completely inhibited ozone-induced increases in PGE recovered by BAL and the suppression of peritoneal macrophage phagocytic activity. The inactive enantiomer of naproxen, l-naproxen, was without effect. Indomethacin partially inhibited ozone-induced suppression of alveolar macrophage phagocytic activity. These observations suggest that prostanoids play a key role in the response to ozone.
持续暴露于臭氧（0.5 ppm，1 - 14天）会降低小鼠肺泡巨噬细胞和腹腔巨噬细胞的吞噬活性。腹腔巨噬细胞对臭氧的反应与肺泡巨噬细胞的反应几乎没有区别。当外源性添加前列腺素E2（PGE2）时，它会抑制肺泡巨噬细胞和腹腔巨噬细胞的吞噬作用。为了检验类前列腺素介导臭氧对巨噬细胞作用的假说，我们测量了支气管肺泡灌洗液（BALF）中的PGE水平以及用环氧化酶抑制剂预处理的臭氧暴露小鼠巨噬细胞的吞噬活性。臭氧暴露后，BALF中的PGE水平升高，高水平的PGE与吞噬活性的大幅降低相关。用吲哚美辛和d - 萘普生预处理可完全抑制臭氧诱导的BAL中回收的PGE增加以及腹腔巨噬细胞吞噬活性的抑制。萘普生的无活性对映体l - 萘普生则没有作用。吲哚美辛部分抑制臭氧诱导的肺泡巨噬细胞吞噬活性的抑制。这些观察结果表明，类前列腺素在对臭氧的反应中起关键作用。
