Carrique-Mas J J, Davies R H
Department of Food and Environmental Safety, Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Rev Sci Tech. 2008 Dec;27(3):665-77. doi: 10.20506/rst.27.3.1829.
The detection of Salmonella in primary poultry production is an issue of great concern in the European Union (EU), since control of this zoonotic disease is in part based on the reduction of the prevalence at the farm level. Success of detection is likely to be highly dependent on the choice of an adequate sampling procedure combined with a sensitive culture method. In poultry farms 'naturally pooled' faeces/litter and dust are the matrices of choice. In floor systems boot swabs are the preferred method for the collection of faeces. A wide range of culture methods is available, but ISO 6579:2002 (Annex D) is currently the standard for poultry environmental samples in the EU. In this review, the authors discuss in detail the range of sampling and culture methodologies for Salmonella in poultry farms. The review also covers sampling and testing of poultry hatcheries, live birds and poultry carcasses.
在家禽初级生产中检测沙门氏菌是欧盟（EU）极为关注的问题，因为对这种人畜共患病的控制部分基于降低农场层面的流行率。检测的成功很可能高度依赖于选择适当的采样程序并结合灵敏的培养方法。在家禽养殖场，“自然混合”的粪便/垫料和灰尘是首选的基质。在地面养殖系统中，靴套拭子是采集粪便的首选方法。有多种培养方法可供选择，但ISO 6579:2002（附录D）目前是欧盟家禽环境样本的标准。在本综述中，作者详细讨论了家禽养殖场中沙门氏菌的采样和培养方法的范围。该综述还涵盖了家禽孵化场、活禽和家禽胴体的采样和检测。
