Granelli K, Elgerud Christina, Lundström Asa, Ohlsson Anita, Sjöberg Pernilla
Chemistry Division 1, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden.
Anal Chim Acta. 2009 Apr 1;637(1-2):87-91. doi: 10.1016/j.aca.2008.08.025. Epub 2008 Aug 28.
A way of carrying out simple and rapid multi-residue analysis of antibiotics in porcine and bovine muscle by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is described. The method has previously been published as a screening method, but the scope has now been extended to quantification and confirmation. Nineteen compounds from five different classes of antibiotics, i.e. tetracyclines, sulfonamides, quinolones, (-lactams and macrolides, are included in the method. The samples are extracted by a single extraction using 70% methanol, diluted with water and injected in the LC-MS/MS. The run time is 7min per injection. About 60 samples can be analysed in 24h. By using this method the need for a separate screening step prior to confirmation is eliminated and consequently the total time from sampling to a confirmed result will be considerably reduced. Validation was performed according to Commission Decision 2002/657/EC.
本文描述了一种通过液相色谱-串联质谱法（LC-MS/MS）对猪和牛肌肉中的抗生素进行简单快速多残留分析的方法。该方法先前已作为一种筛查方法发表，但现在其范围已扩展到定量和确证。该方法涵盖了来自五类不同抗生素的19种化合物，即四环素类、磺胺类、喹诺酮类、β-内酰胺类和大环内酯类。样品通过使用70%甲醇进行单次萃取，用水稀释后注入LC-MS/MS。每次进样的运行时间为7分钟。24小时内可分析约60个样品。通过使用该方法，消除了确证前单独进行筛查步骤的需求，因此从采样到获得确证结果的总时间将大大缩短。根据欧盟委员会第2002/657/EC号决定进行了方法验证。
