Nielen M W F, Hooijerink H, Claassen F C, van Engelen M C, van Beek T A
RIKILT Institute of Food Safety, P.O. Box 230, 6700 AE Wageningen, The Netherlands.
Anal Chim Acta. 2009 Apr 1;637(1-2):92-100. doi: 10.1016/j.aca.2008.08.036. Epub 2008 Sep 3.
Hormone and veterinary drug screening and forensics can benefit from the recent developments in desorption electrospray ionisation (DESI) mass spectrometry (MS). In this work the feasibility of DESI application has been studied. Using a linear ion trap or quadrupole time-of-flight (TOF) MS instrument both full-scan and data-dependent collision-induced dissociation MS(n) spectra were acquired in seconds without sample preparation. Preliminary data are presented for the rapid screening of (pro)hormone supplement samples, an illegal steroid cocktail and forensic samples from veterinary drug investigations. The potential of this DESI approach is clearly demonstrated since compounds observed could be independently confirmed by liquid chromatography/TOFMS with accurate mass measurement, and/or proton nuclear magnetic resonance spectroscopy. Specific concerns related to false-positive and false-negative findings due to limitations in quantification and memory-effects are briefly discussed. It is envisaged that DESI will achieve a prominent role in hormone and veterinary drug analysis in the near future.
激素和兽药筛查以及法医学可受益于解吸电喷雾电离（DESI）质谱（MS）的最新进展。在这项工作中，对DESI应用的可行性进行了研究。使用线性离子阱或四极杆飞行时间（TOF）质谱仪，无需样品制备，即可在数秒内获得全扫描和数据依赖型碰撞诱导解离MS(n)光谱。给出了对（前体）激素补充剂样品、非法类固醇混合物以及兽药调查中的法医样品进行快速筛查的初步数据。由于所观察到的化合物可通过液相色谱/TOFMS进行精确质量测量和/或质子核磁共振光谱进行独立确认，因此该DESI方法的潜力得到了明确证明。简要讨论了由于定量限制和记忆效应导致的假阳性和假阴性结果的具体问题。预计DESI在不久的将来将在激素和兽药分析中发挥重要作用。
