Surovaya Olga V, Dosenko Victor E, Nagibin Vasyl S, Tumanovskaya Lesya V, Moibenko Alexey A
Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, 01024 Kiev, Ukraine.
Pathophysiology. 2009 Jun;16(1):47-52. doi: 10.1016/j.pathophys.2009.01.012. Epub 2009 Mar 14.
The cell death and gene expression in neonatal cardiomyocyte cultures were investigated in a late postconditioning model. The primary cultures were subjected to a 30min of anoxia followed by 60min or 24h of reoxygenation. Postconditioning was carried out in three cycles of 1min reoxygenations followed by 1min anoxia, respectively. After 24h of reperfusion the percentages of living, necrotic, and apoptotic cells were determined by staining with bis-benzimide and propidium iodide. Anoxia-reoxygenation significantly increased the necrotic and apoptotic cells both at its first and second episodes. Postconditioning in remote period did not protect the cells from the second anoxia. Postconditioning decreased the anoxia-reoxygenation-induced increase of HSP70 and HSP90 mRNA expression. We observed a decrease of HIF-3alpha gene expression in remote postconditioning. The FRAP gene expression was leveled to control value. Thus, the changes of mRNA gene expression did not show cytoprotection of cardiomyocytes in remote postconditioning model.
在晚期后适应模型中研究了新生心肌细胞培养物中的细胞死亡和基因表达。原代培养物先经历30分钟缺氧，然后再进行60分钟或24小时复氧。后适应分别通过三个1分钟复氧和1分钟缺氧的循环进行。再灌注24小时后，通过用双苯甲酰亚胺和碘化丙啶染色来测定存活、坏死和凋亡细胞的百分比。缺氧-复氧在其第一次和第二次发作时均显著增加了坏死和凋亡细胞。远期后适应并不能保护细胞免受第二次缺氧的影响。后适应降低了缺氧-复氧诱导的HSP70和HSP90 mRNA表达的增加。我们观察到远期后适应中HIF-3α基因表达降低。FRAP基因表达恢复到对照值。因此，在远期后适应模型中，mRNA基因表达的变化未显示对心肌细胞的细胞保护作用。
