Kwong-Hing A, Teasdale R, Sandhu H S
Division of Periodontics and Oral Biology, Faculty of Dentistry, University of Western Ontario, London, Canada.
Anat Embryol (Berl). 1991;184(1):93-7. doi: 10.1007/BF01744265.
To study the relationship between the mechanical properties of collagen and the bone turnover, 2-week-old Balb/C mice were fed on a diet containing 0.25% Beta aminopropionitrile (B-APN), a potent inhibitor of collagen crosslink formation, for 3 weeks. Mandibular incisor socket was selected for the analysis of bone formation and resorption parameters. Plastic embedded sections stained with toluidine blue and cut at 4 microns were used to analyze the average area of bone lamellae, bone-forming surface, and the number of osteoblasts/mm of forming surface. Similar sections were used to localize acid phosphatase on resorbing surfaces and within the osteoclasts, while bone alkaline phosphatase was determined by a colorimetric method. Morphometric analyses showed that the area of newly formed bone lamellae, total forming surface, number of osteoblasts and the Alk. Pase activity were significantly lower in B-APN-fed mice as compared to the controls. There was a concomitant smaller, but significant, reduction in total resorption surface, active resorption surface and the number of osteoclasts. These results suggest that the regulation of bone formation and resorption at this site, which is independent of systemic regulation, is influenced by the mechanical properties of the collagenous matrix, which in turn may have a significant effect on the existing pool of bone-forming cells, but may not influence the recruitment of new cells.
为研究胶原蛋白的力学性能与骨转换之间的关系，给2周龄的Balb/C小鼠喂食含0.25%β-氨基丙腈（B-APN，一种有效的胶原蛋白交联形成抑制剂）的饲料，持续3周。选择下颌切牙牙槽窝分析骨形成和吸收参数。用甲苯胺蓝染色并切成4微米厚的塑料包埋切片用于分析骨板平均面积、骨形成表面以及每毫米形成表面的成骨细胞数量。类似的切片用于在吸收表面和破骨细胞内定位酸性磷酸酶，而骨碱性磷酸酶则通过比色法测定。形态计量分析表明，与对照组相比，喂食B-APN的小鼠中新形成的骨板面积、总形成表面、成骨细胞数量和碱性磷酸酶活性显著降低。总吸收表面、活跃吸收表面和破骨细胞数量也随之出现较小但显著的减少。这些结果表明，该部位的骨形成和吸收调节独立于全身调节，受胶原基质力学性能的影响，这反过来可能对现有的成骨细胞池有显著影响，但可能不影响新细胞的募集。
