Sandhu H S, Jande S S
Acta Anat (Basel). 1981;111(4):281-8. doi: 10.1159/000145478.
Bones from 2-week-old chicks, fed a diet containing 0.05% beta-aminopropionitrile (beta-APN), were studied for 3H-tetracycline and 3H-proline incorporation at 7, 14 and 21 days of the experiment. Liquid scintillation counts showed a comparable amount of 3H-proline incorporation in the controls and experimentals. However, the incorporation of 3H-tetracycline was significantly lower in experimentals at all intervals. Fluorescent microscopy after cold tetracycline injections at similar intervals showed a comparable amount of linear bone apposition in controls and beta-APN-fed chicks. The bone ash content in beta-APN-fed chicks was lower at all intervals whereas the serum calcium level was similar to the controls. Microradiography showed that cortical and marrow areas, in cross sections, were not significantly different in the controls and beta-APN-fed chicks. On the basis of these data it is suggested that beta-APN at the dose used does not inhibit the bone matrix synthesis but inhibits its mineralization.
对喂食含0.05%β-氨基丙腈（β-APN）日粮的2周龄雏鸡的骨骼，在实验的第7、14和21天研究了3H-四环素和3H-脯氨酸的掺入情况。液体闪烁计数显示，对照组和实验组中3H-脯氨酸的掺入量相当。然而，在所有时间段，实验组中3H-四环素的掺入量均显著较低。在相似时间段注射冷四环素后进行荧光显微镜检查，结果显示对照组和喂食β-APN的雏鸡中线性骨沉积量相当。在所有时间段，喂食β-APN的雏鸡的骨灰含量均较低，而血清钙水平与对照组相似。显微放射照相显示，对照组和喂食β-APN的雏鸡的皮质和骨髓区域在横切面上无显著差异。基于这些数据，表明所用剂量的β-APN不抑制骨基质合成，但抑制其矿化。
