Yang Jianli, Wang Ji, Zhao Jia, Zuo Dan, Li Xingrong, Wang Li
Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun, PR China.
Oncol Rep. 2009 Apr;21(4):949-55. doi: 10.3892/or_00000308.
Basic fibroblast growth factor (bFGF) is closely involved in angiogenesis and tumor growth of various cancers, but its role in cervical cancer remains to be defined. We investigated the effects of bFGF on HeLa cell growth and studied its influence on the expression of angiogenin. We transfected the bFGF gene in the sense and antisense orientation into HeLa cells, and obtained stable bFGF underexpressing and overexpressing transfectants. In our experiments, we demonstrated that inhibition of bFGF gene and protein expression in the bFGF antisense transfectants induced increased protein expression of angiogenin. In contrast, in the bFGF sense transfectants the expression of angiogenin decreased. Delivery of recombinant angiogenin into transfected and control cells led to increased proliferation in the bFGF antisense transfectants and the control cells. However, the cell proliferation had no change in the bFGF sense transfectants. In conclusion, we demonstrated that besides its angiogenic activity, bFGF and angiogenin also directly contribute to HeLa cell proliferation. Furthermore, endogenous bFGF affects the expression of angiogenin in HeLa cells. These findings suggest that inhibition of bFGF alone is not a promising strategy to inhibit angiogenesis.
碱性成纤维细胞生长因子（bFGF）与多种癌症的血管生成和肿瘤生长密切相关，但其在宫颈癌中的作用仍有待明确。我们研究了bFGF对HeLa细胞生长的影响，并探讨了其对血管生成素表达的影响。我们将有义及反义方向的bFGF基因转染入HeLa细胞，获得了稳定的bFGF低表达和过表达转染细胞株。在我们的实验中，我们证明bFGF反义转染细胞株中bFGF基因和蛋白表达的抑制导致血管生成素蛋白表达增加。相反，在bFGF有义转染细胞株中血管生成素的表达降低。将重组血管生成素导入转染细胞和对照细胞导致bFGF反义转染细胞株和对照细胞的增殖增加。然而，bFGF有义转染细胞株中的细胞增殖没有变化。总之，我们证明除了其血管生成活性外，bFGF和血管生成素还直接促进HeLa细胞增殖。此外，内源性bFGF影响HeLa细胞中血管生成素的表达。这些发现表明，单独抑制bFGF并非抑制血管生成的有效策略。
