Wang Ji, Yang Jianli, Yuan Dawei, Wang Jun, Zhao Jia, Wang Li
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
J Genet Genomics. 2009 Jul;36(7):399-407. doi: 10.1016/S1673-8527(08)60129-0.
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Basic fibroblast growth factor (bFGF), which is highly expressed in developing tissues and malignant cells, regulates cell growth, differentiation, and migration. Its expression is essential for the progression and metastasis of HCC. This study aims to investigate the effects of bFGF on the expression of angiogenin, another growth factor, which plays an important role in tumor angiogenesis, and on cell proliferation in H7402 human hepatoma cells. The bFGF sense cDNA or antisense cDNA was stably transfected into H7402 cells. Genomic DNA PCR analysis demonstrated that human bFGF sense cDNA or antisense cDNA was inserted into the genome. Furthermore, the expression of bFGF and angiogenin was examined by RT-PCR and Western blot assays. MTT and colony formation assays were employed to determine cell proliferation. Stable bFGF over-expressing and under-expressing transfectants were successfully established. Expression of angiogenin was decreased in the over-expressing bFGF cells (sense transfectants) and was increased in the under-expressing bFGF cells (antisense transfectants). Cell proliferation increased in the bFGF sense transfectants and decreased in the bFGF antisense transfectants. These results demonstrated that the endogenous bFGF may not only negatively regulate the angiogenin expression but also contribute to the overall cell proliferation in H7402 human hepatoma cells. This study may be helpful in finding a potential therapeutic approach to HCC.
肝细胞癌（HCC）是全球最常见的癌症之一。碱性成纤维细胞生长因子（bFGF）在发育中的组织和恶性细胞中高度表达，可调节细胞生长、分化和迁移。其表达对于HCC的进展和转移至关重要。本研究旨在探讨bFGF对血管生成素（另一种在肿瘤血管生成中起重要作用的生长因子）表达以及对H7402人肝癌细胞增殖的影响。将bFGF正义cDNA或反义cDNA稳定转染至H7402细胞中。基因组DNA PCR分析表明人bFGF正义cDNA或反义cDNA已插入基因组。此外，通过RT-PCR和蛋白质印迹分析检测bFGF和血管生成素的表达。采用MTT和集落形成试验来确定细胞增殖。成功建立了稳定的bFGF过表达和低表达转染细胞系。血管生成素的表达在bFGF过表达细胞（正义转染细胞）中降低，而在bFGF低表达细胞（反义转染细胞）中升高。bFGF正义转染细胞中细胞增殖增加，而bFGF反义转染细胞中细胞增殖减少。这些结果表明内源性bFGF可能不仅负向调节血管生成素的表达，而且对H7402人肝癌细胞的总体细胞增殖有贡献。本研究可能有助于找到一种潜在的HCC治疗方法。
