Piccone M E, Pauszek S, Pacheco J, Rieder E, Kramer E, Rodriguez L L
Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, USDA/ARS/NAA, Greenport, NY, 11944-0848, USA.
Arch Virol. 2009;154(4):671-6. doi: 10.1007/s00705-009-0332-x. Epub 2009 Mar 14.
A foot-and-mouth disease virus containing a 57-nucleotide (nt) insertion in the 3'untranslated region (3'UTR) was generated by transposon (tn)-mediated mutagenesis. Characterization of the mutant virus (A24-3'UTR8110) revealed no significant differences in virus growth, translation efficiency or virulence in cattle compared to the A24 wild-type virus. RNA modeling showed that the structures predicted in the 3'UTR were not affected by the tn insertion. These results revealed that the 3'UTR can tolerate foreign sequences that do not disrupt essential signals required for virus replication.
通过转座子（tn）介导的诱变产生了一种在3'非翻译区（3'UTR）含有57个核苷酸（nt）插入片段的口蹄疫病毒。对突变病毒（A24-3'UTR8110）的特性分析表明，与A24野生型病毒相比，其在牛体内的病毒生长、翻译效率或毒力方面没有显著差异。RNA建模显示，3'UTR中预测的结构不受tn插入的影响。这些结果表明，3'UTR能够耐受不破坏病毒复制所需基本信号的外源序列。
