Li Yu-Qin, Xue Tao, Wang Liang, Xu Zu-Cai, Xi Zhi-Qin, Yuan Jie, Wang Xue-Feng, Chen Yang-Mei, Zhang Meilin, Yao Lan
Department of Neurology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Neurochem Res. 2009 Sep;34(9):1594-602. doi: 10.1007/s11064-009-9948-1. Epub 2009 Mar 14.
Epithelial membrane protein-1 (EMP-1), called Tumor-associated membrane protein, is the marker of a drug-resistant tumor and take part in the drug-resistant mechanism of tumor, with the relationship of epidermal growth factor receptor (EGFR). Because there are some similarities between the pathogenesis and the drug resistance mechanism of tumor and the drug resistance mechanisms in epilepsy. EMP1 expression may be connected with the drug-resistance mechanism of epilepsy. We detected EMP-1 by gene scanning and immunohistochemistry staining, comparing the IE group and the control group, and we investigated the relationship between EMP-1 and EGFR by double-label immunofluorescence staining in the IE group. We found expression of EMP-1 mRNA was higher in IE per the gene scanning, EMP-1 immunoreactivity was apparent in neurons of IE patients but not in the control group, and the expression of EMP-1 and EGFR occurred in the same neuron. We confirm EMP-1 is abnormally expressed in IE and suggest the interaction of EGFR and EMP-1 plays a role in the mechanism of drug resistance in epilepsy and may be a new gene for drug resistance.
上皮膜蛋白-1（EMP-1），也被称为肿瘤相关膜蛋白，是耐药肿瘤的标志物，参与肿瘤的耐药机制，与表皮生长因子受体（EGFR）有关。由于肿瘤的发病机制和耐药机制与癫痫的耐药机制存在一些相似之处。EMP1的表达可能与癫痫的耐药机制有关。我们通过基因扫描和免疫组织化学染色检测EMP-1，比较颞叶癫痫（IE）组和对照组，并通过双标免疫荧光染色在IE组中研究EMP-1与EGFR之间的关系。我们发现，通过基因扫描，IE中EMP-1 mRNA的表达更高，EMP-1免疫反应性在IE患者的神经元中明显，但在对照组中不明显，并且EMP-1和EGFR的表达出现在同一神经元中。我们证实EMP-1在IE中异常表达，并表明EGFR和EMP-1的相互作用在癫痫耐药机制中起作用，可能是一个新的耐药基因。
