Oral Cancer Research Institute, Yonsei University College of Dentistry, Seoul, South Korea.
Phytother Res. 2009 Sep;23(9):1326-31. doi: 10.1002/ptr.2540.
In this study, sophoraflavanone G obtained from Sophora flavescens was evaluated against 10 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA), either alone or in combination with ampicillin or oxacillin, via checkerboard assay. At the end point of an optically clear well, the minimum inhibitory concentrations (MICs) ranged from 0.5 to 8 microg/ml for sophoraflavanone G, from 64 to 1024 microg/ml for ampicillin, and from 256 to 1024 microg/ml for oxacillin. The combination of sophoraflavanone G and ampicillin or oxacillin yielded a fractional inhibitory concentration index ranging from 0.188 to 0.375, thereby indicating a principally synergistic effect. The synergistic interaction was verified by time-kill studies using sophoraflavanone G and/or antibiotics. Thirty minutes of treatment with sophoraflavanone G with ampicillin or oxacillin resulted in an increase in the rate of killing in units of CFU/ml to a greater degree than was observed with Sophoraflavanone G alone. These findings indicated that the application of the tested sophoraflavanone G alone or in combination with antibiotics might prove useful in the control and treatment of MRSA infections.
在这项研究中，从苦参中获得的槐黄酮 G 与氨苄西林或苯唑西林单独或联合使用，通过棋盘微量稀释法对 10 株耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株进行了评估。在光学透明孔的终点处，槐黄酮 G 的最小抑菌浓度（MIC）范围为 0.5 至 8μg/ml，氨苄西林的 MIC 范围为 64 至 1024μg/ml，苯唑西林的 MIC 范围为 256 至 1024μg/ml。槐黄酮 G 与氨苄西林或苯唑西林联合使用时，部分抑菌浓度指数（FICI）范围为 0.188 至 0.375，表明主要具有协同作用。协同相互作用通过使用槐黄酮 G 和/或抗生素的时间杀伤研究得到验证。用槐黄酮 G 联合氨苄西林或苯唑西林处理 30 分钟，与单独使用槐黄酮 G 相比，以 CFU/ml 为单位的杀菌率增加程度更大。这些发现表明，测试的槐黄酮 G 单独或与抗生素联合应用可能有助于控制和治疗 MRSA 感染。
