Zwirchmayr Julia, Cruz Cristina D, Grienke Ulrike, Tammela Päivi, Rollinger Judith M
Department of Pharmaceutical Sciences, Division of Pharmacognosy, Faculty of Life Sciences, University of Vienna, 1090 Vienna, Austria.
Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, 00014 Helsinki, Finland.
iScience. 2023 Aug 3;26(9):107523. doi: 10.1016/j.isci.2023.107523. eCollection 2023 Sep 15.
The root extract of (PO-E) was identified as a promising antibacterial source from a screening of 158 extracts against . It has also recently been shown to significantly decrease the survival of the nematode . We used the biochemometric approach ELINA to investigate the phytochemical characteristics of the multicomponent mixture PO-E to identify the anti-infective constituent(s) targeting and H NMR spectra of PO-E-derived microfractions were correlated with their respective bioactivity data. Heterocovariance analyses unambiguously identified ostruthin as an anti-staphylococcal constituent, which potently also inhibited spp.. ELINA demonstrated that anthelmintic activity was due to a combinatorial effect of ostruthin and isoimperatorin. A -based survival and motility assay confirmed that isoimperatorin, imperatorin, and verapamil modulated the susceptibility of ostruthin. The combinatorial effect of these natural products was shown in larvae studies to be related to the function of the nematodes' efflux pump.
从对158种提取物针对[具体对象未明确]的筛选中，植物名称未明确的根提取物被确定为一种有前景的抗菌来源。最近还显示它能显著降低线虫的存活率。我们使用生物计量学方法ELINA来研究多组分混合物PO-E的植物化学特征，以鉴定针对[具体对象未明确]的抗感染成分，并将PO-E衍生的微馏分的1H NMR光谱与其各自的生物活性数据相关联。异协方差分析明确鉴定出蛇床素是一种抗葡萄球菌成分，它也能有效抑制[具体菌种未明确]。ELINA表明驱虫活性是由于蛇床素和异欧前胡素的组合效应。基于[具体实验未明确]的存活和运动性测定证实，异欧前胡素、欧前胡素和维拉帕米调节了蛇床素的敏感性。在幼虫研究中表明，这些天然产物的组合效应与线虫外排泵的功能有关。
