Wang Zi-jian, Wu Xiu-ping, Deng Hong-kuan, Liu Ming-yuan
Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, Changchun 130062, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2008 Dec 30;26(6):476-9.
RNA interference is a powerful tool for investigating gene function which has been used extensively in model organisms such as Caenorhabditis elegans and has been adapted as a high-throughput screening method to identify genes. However, recent research indicates that the application of RNAi to animal parasitic nematodes has some problems. For example, the variability of RNAi to different parasitic nematodes or the same nematode in different stages is great. Reasons to explain why RNAi does not work well in animal parasitic nematodes include: (1) the efficacy of RNAi delivery methods in different nematodes is extremely variable; (2) RNAi mechanism has been applied to related nematodes and most of them are gene functional defect; (3) different lifestyles of nematodes may influence on the efficacy of RNAi. If we can find out the methods which can solve these questions, there are still application prospects for using RNAi in parasitic nematodes with improved RNAi effect.
RNA干扰是研究基因功能的有力工具，已在秀丽隐杆线虫等模式生物中广泛应用，并已被改编为一种高通量筛选方法来鉴定基因。然而，最近的研究表明，RNA干扰应用于动物寄生线虫存在一些问题。例如，RNA干扰对不同寄生线虫或同一线虫不同阶段的变异性很大。解释RNA干扰在动物寄生线虫中效果不佳的原因包括：（1）RNA干扰递送方法在不同线虫中的效果差异极大；（2）RNA干扰机制已应用于相关线虫，且大多数是基因功能缺陷；（3）线虫的不同生活方式可能会影响RNA干扰的效果。如果我们能找到解决这些问题的方法，那么提高RNA干扰效果后，RNA干扰在寄生线虫中的应用仍有前景。
