Poffenbarger E M, Olson P N, Chandler M L, Seim H B, Varman M
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Am J Vet Res. 1991 Aug;52(8):1221-4.
Failure to obtain passive transfer of immunity via colostrum can be detrimental to the health and survival of a young pup. It has been stated that pups that do not receive colostrum in the first 2 days after birth, be given adult dog serum as a source of protective immunoglobulins. Twenty-five Beagle pups were obtained by cesarean section from 6 Beagle bitches. The pups were allotted to 3 groups at birth. Group 1 was a control group and was allowed to suckle colostrum. Group-2 pups received 22 ml of pooled adult dog serum/kg of body weight (10 ml/lb) SC at birth. Group-3 pups were given 22 ml of pooled adult dog serum/kg by stomach tube at birth. Pups from groups 2 and 3 were separated from the bitch for 48 hours to prevent colostral antibody absorption and were fed a commercially available milk replacer by stomach tube. After 48 hours, all pups were returned to the bitch until they were weaned at 6 weeks of age. Blood samples were collected from all of the pups at birth and on days 1, 2, 7, 14, 21, 28, and 35. The concentration of IgA, IgG, and IgM in serum was determined by radial immunodiffusion and compared by use of a one-way analysis of variance. The control pups had significantly higher serum concentrations of IgA and IgG, than the pups in groups 2 and 3 on days 1 and 2 and 2 and 7, respectively. Group-2 pups had significantly higher serum IgM concentrations on day 1 than either group 1- or group-3 pups.
未能通过初乳获得免疫的被动转移可能对幼犬的健康和存活有害。有人指出，出生后前两天未摄入初乳的幼犬，应给予成年犬血清作为保护性免疫球蛋白的来源。通过剖宫产从6只比格犬母犬获得了25只比格幼犬。幼犬出生时被分为3组。第1组为对照组，允许其吸食初乳。第2组幼犬在出生时皮下注射22毫升/千克体重（10毫升/磅）的成年犬混合血清。第3组幼犬在出生时通过胃管给予22毫升/千克的成年犬混合血清。将第2组和第3组的幼犬与母犬分开48小时，以防止初乳抗体吸收，并通过胃管喂食市售的代乳品。48小时后，所有幼犬都回到母犬身边，直到6周龄断奶。在出生时以及出生后第1、2、7、14、21、28和35天从所有幼犬采集血样。通过放射免疫扩散法测定血清中IgA、IgG和IgM的浓度，并使用单向方差分析进行比较。对照组幼犬在出生后第1天和第2天以及第2天和第7天的血清IgA和IgG浓度分别显著高于第2组和第3组的幼犬。第2组幼犬在第1天的血清IgM浓度显著高于第1组和第3组的幼犬。
