Mitani Tomoaki, Akane Atsushi, Tokiyasu Takuma, Yoshimura Sumitaka, Okii Yutaka, Yoshida Manabu
Department of Legal Medicine, Kansai Medical University, Osaka 570-8506, Japan.
Leg Med (Tokyo). 2009 Apr;11 Suppl 1:S449-50. doi: 10.1016/j.legalmed.2009.02.002. Epub 2009 Mar 16.
This study investigated a PCR direct sequencing method for species identification by analyzing partial sequences in the mitochondrial 16S rRNA genes of many animal species amplified with universal primers. Samples from 182 vertebrates and 103 invertebrates were analyzed, and the sequences could be obtained in 182 and 72 species, respectively. The sequence divergence was sufficient to identify the species at the level of genus with the aid of the GenBank database and the BLAST tool. This method could be a powerful tool for animal species identification, especially in forensic cases in which many unknown biological samples should be analyzed.
本研究通过分析用通用引物扩增的多种动物线粒体16S rRNA基因的部分序列，对一种用于物种鉴定的PCR直接测序方法进行了研究。分析了来自182种脊椎动物和103种无脊椎动物的样本，分别在182种和72种物种中获得了序列。借助GenBank数据库和BLAST工具，序列差异足以在属水平上鉴定物种。该方法可能是动物物种鉴定的有力工具，特别是在需要分析许多未知生物样本的法医案件中。
