Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Italy.
Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, Bologna, Italy.
Int J Legal Med. 2021 Mar;135(2):387-392. doi: 10.1007/s00414-020-02388-9. Epub 2020 Aug 11.
Genetic testing of animal biological material has become a valuable tool in forensic investigations, and it is successfully used to identify unknown crime perpetrators, to unmask food frauds, or to clarify cases of animal attacks on humans or other animals. When DNA profiling is not possible due to inadequate amounts of nuclear DNA, mitochondrial DNA (mtDNA) testing is the only viable alternative, as in the case of shed hair samples. In this case, a dog was allegedly killed by wild animals while being hosted in a boarding house. Extraneous hair fragments recovered from the dog's mouth and paws were subjected to genetic analysis: the cytochrome b gene located on mtDNA was amplified and sequenced in order to determine the species responsible for the killing. The mtDNA analysis provided evidence that the dog was killed by other dogs, thus unmasking a false wild animal attack and putting the case in an entirely different perspective.
对动物生物材料进行基因检测已成为法医学调查中的一项有价值的工具，它成功地用于识别未知的犯罪嫌疑人、揭露食品欺诈行为，或澄清涉及人类或其他动物的动物袭击案件。当由于核 DNA 含量不足而无法进行 DNA 图谱分析时，线粒体 DNA（mtDNA）检测是唯一可行的替代方法，例如在脱落毛发样本的情况下。在这种情况下，一只据称在寄宿旅馆中被野生动物杀死的狗。从狗的嘴和爪子上提取的外源毛发碎片进行了基因分析：位于 mtDNA 上的细胞色素 b 基因被扩增和测序，以确定负责杀戮的物种。mtDNA 分析提供了证据，证明狗是被其他狗杀死的，从而揭露了一起虚假的野生动物袭击事件，并使案件呈现出完全不同的视角。
