Pilli Elena, Casamassima Rosario, Vai Stefania, Virgili Antonino, Barni Filippo, D'Errico Giancarlo, Berti Andrea, Lago Giampietro, Caramelli David
Dipartimento di Biologia, Università di Firenze, via del Proconsolo 12, 50122 Florence, Italy.
Reparto Carabinieri Investigazioni Scientifiche di Roma, Sezione di Chimica, viale Tor di Quinto 119, Rome, Italy.
Investig Genet. 2014 Jun 2;5:7. doi: 10.1186/2041-2223-5-7. eCollection 2014.
In forensic science there are many types of crime that involve animals. Therefore, the identification of the species has become an essential investigative tool. The exhibits obtained from such offences are very often a challenge for forensic experts. Indeed, most biological materials are traces, hair or tanned fur. With hair samples, a common forensic approach should proceed from morphological and structural microscopic examination to DNA analysis. However, the microscopy of hair requires a lot of experience and a suitable comparative database to be able to recognize with a high degree of accuracy that a sample comes from a particular species and then to determine whether it is a protected one. DNA analysis offers the best opportunity to answer the question, 'What species is this?' In our work, we analyzed different samples of fur coming from China used to make hats and collars. Initially, the samples were examined under a microscope, then the mitochondrial DNA was tested for species identification. For this purpose, the genetic markers used were the 12S and 16S ribosomal RNA, while the hypervariable segment I of the control region was analyzed afterwards, to determine whether samples belonged to the same individual.
Microscopic examination showed that the fibres were of animal origin, although it was difficult to determine with a high degree of confidence which species they belonged to and if they came from a protected species. Therefore, DNA analysis was essential to try to clarify the species of these fur samples.
Macroscopic and microscopic analysis confirmed the hypothesis regarding the analyzed hair belonging to real animals, although it failed to prove with any kind of certainty which actual family it came from, therefore, the species remains unknown. Sequence data analysis and comparisons with the samples available in GenBank showed that the hair, in most cases, belonged to the Canidae family, and in one case only to Felidae.
在法医学中,有许多涉及动物的犯罪类型。因此,物种鉴定已成为一项重要的调查工具。从这类犯罪中获取的证物对法医专家来说往往是一项挑战。实际上,大多数生物材料都是痕迹、毛发或鞣制过的皮毛。对于毛发样本,常见的法医鉴定方法应从形态和结构的显微镜检查到DNA分析。然而,毛发的显微镜检查需要很多经验和合适的比较数据库,以便能够高度准确地识别一个样本来自特定物种,然后确定它是否为受保护物种。DNA分析为回答“这是什么物种?”这个问题提供了最佳机会。在我们的工作中,我们分析了来自中国的用于制作帽子和衣领的不同皮毛样本。最初,在显微镜下检查样本,然后测试线粒体DNA以进行物种鉴定。为此,使用的基因标记是12S和16S核糖体RNA,随后分析控制区的高变区I,以确定样本是否属于同一个体。
显微镜检查表明纤维是动物来源的,尽管很难高度确信地确定它们属于哪个物种以及是否来自受保护物种。因此,DNA分析对于试图厘清这些皮毛样本的物种至关重要。
宏观和微观分析证实了关于所分析毛发属于真实动物的假设,尽管未能确定地证明它实际来自哪个科,因此,物种仍然未知。序列数据分析以及与GenBank中可用样本的比较表明,这些毛发在大多数情况下属于犬科,仅在一个案例中属于猫科。