斯氏线虫从含有番茄种子的藻酸盐胶囊中逸出。
Escape of Steinernema feltiae from Alginate Capsules Containing Tomato Seeds.
作者信息
Kaya H K, Mannion C M, Burlando T M, Nelsen C E
出版信息
J Nematol. 1987 Jul;19(3):287-91.
The entomogenous nematode Steinernema feltiae was encapsulated in an alginate matrix containing a tomato seed. When these capsules were placed on 0.8% agar for 7 days, the seed germinated and ca. 20% of the nematodes escaped from the capsules, whereas only 0.1% escaped from capsules without seeds. When capsules containing nematodes and a seed were planted into sterilized or nonsterilized soil, nematodes escaped to infect Galleria mellonella larvae. When seed in capsules containing ca. 274 nematodes per capsule were planted in nonsterilized soil, Galleria mortality was 90% 1 week later. Galleria mortality declined to 27%, 23%, and 0% in weeks 2, 4, and 8 postplant, respectively. In sterilized soil, Galleria mortality was 96% and did not differ significantly from the nonsterilized soil in week 1, but was significantly higher in sterilized soil over nonsterilized soil for week 2 (81%) and week 4 (51%). When capsules containing nematodes only were used, Galleria mortality was 71% in sterilized soil 1 week after planting and 58%, 33%, and 12% in weeks 2, 4, and 8 postplant, respectively. In nonsterilized soil, Galleria mortality was 8%, 30%, 21%, and 28% after 1, 2, 4, and 8 weeks, respectively, using only encapsulated nematodes. When the number of nematodes per capsule was increased to ca. 817, Galleria mortality was 92 % or higher in sterilized soil from week 1 to week 4.
昆虫病原线虫斯氏线虫被包裹在含有番茄种子的藻酸盐基质中。当这些胶囊置于0.8%的琼脂上7天时，种子发芽，约20%的线虫从胶囊中逸出，而没有种子的胶囊中只有0.1%的线虫逸出。当含有线虫和种子的胶囊被种植到灭菌或未灭菌的土壤中时，线虫逸出以感染大蜡螟幼虫。当每个胶囊含有约274条线虫的种子被种植在未灭菌的土壤中时，1周后大蜡螟的死亡率为90%。在种植后的第2、4和8周，大蜡螟的死亡率分别降至27%、23%和0%。在灭菌土壤中，大蜡螟的死亡率为96%，在第1周与未灭菌土壤没有显著差异，但在第2周（81%）和第4周（51%），灭菌土壤中的死亡率显著高于未灭菌土壤。当仅使用含有线虫的胶囊时，种植1周后灭菌土壤中大蜡螟的死亡率为71%，在种植后的第2、4和8周分别为58%、33%和12%。在未灭菌土壤中，仅使用包裹线虫时，1、2、4和8周后的大蜡螟死亡率分别为8%、30%、21%和28%。当每个胶囊中的线虫数量增加到约817条时，从第1周到第4周，灭菌土壤中大蜡螟的死亡率为92%或更高。
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