Ishibashi N, Kondo E
J Nematol. 1986 Jul;18(3):310-6.
Infective juveniles (J3) of the entomogenous nematodes Steinernema feltiae DD-136 (ca. 10,000 J3/100 ml) and S. glaseri (ca. 2,500 J3/100 ml) were incubated in steam-sterilized and nonsterilized sandy soil and bark compost for 8 weeks at 25 C. The nematodes were recovered by a two-step extraction procedure at 1-week intervals, and their infectivity to lepidopterous larvae (Spodoptera litura and Galleria mellonella) and their effect on the population and community of native nematodes in soil were determined. Survival of inoculated nematodes and mortality of insects were enhanced in sterilized media. Nonsterilized bark compost proved to be equally as suitable a medium as sterilized compost. In nonsterilized soil, the survival curve of S.feltiae declined more rapidly than that or S. glaseri which was less infective to insects despite its greater persistence even in nonsterilized soil. Soon after the addition of steinernematids to soil, the population of native nematodes showed a fluctuation with an increase in rhabditids and a decrease in other kinds of nematodes.
将昆虫病原线虫斯氏线虫 DD - 136（约 10,000 条感染性幼虫/100 毫升）和格氏斯氏线虫（约 2,500 条感染性幼虫/100 毫升）的感染性幼虫（J3）在 25℃下于蒸汽灭菌和未灭菌的沙质土壤及树皮堆肥中孵育 8 周。每隔 1 周通过两步提取程序回收线虫，并测定它们对鳞翅目幼虫（斜纹夜蛾和米蛾）的感染力及其对土壤中天然线虫种群和群落的影响。在灭菌培养基中，接种线虫的存活率和昆虫的死亡率有所提高。事实证明，未灭菌的树皮堆肥与灭菌堆肥一样适合作为培养基。在未灭菌的土壤中，斯氏线虫 DD - 136 的存活曲线下降速度比格氏斯氏线虫更快，尽管格氏斯氏线虫在未灭菌土壤中的持久性更强，但对昆虫的感染力较低。在向土壤中添加斯氏线虫后不久，天然线虫种群出现波动，其中小杆线虫数量增加，其他种类的线虫数量减少。
