Vyas-Patel N
J Nematol. 1988 Jan;20(1):96-101.
The ability of Romanomermis culicivorax to infect, develop, and emerge from Kenyan mosquito hosts was evaluated in the laboratory. Host species tested were Aedes aegypti, Ae. dentatus, Ae. hirsutus, Anopheles arabiensis, An. coustani, An. funestus, An. gambiae, An. pharoensis, Culex duttoni, Cu. ethiopicus, Cu. poicilipes, Cu. quinquefasciatus, Cu. tigripes, Cu. univittatus, Coquillettidia metallica, Mansonia africana, Ma. uniformis, Mimomyia splendens, Mi. uniformis, Toxorhyncites brevipalpis, and Uranotaenia balfouri. R. culicivorax penetrated all the host species tested and developed and emerged from most of the hosts. Both penetration and some development, but not nematode emergence, were observed from all instars of Ma. uniformis. T. brevipalpis exhibited signs of resistance in the form of melanization of R. culicivorax within 48 hours of infection in all four instar stages. Nematode melanization, especially in older hosts, was observed in Ae. dentatus, Ae. hirsutus, Cu. duttoni, Cu. tigripes, and Mi. splendens. When melanization occurred, the melanized carcass of the nematode was passed on from instar to instar. The implications for field release of R. culicivorax in Kenya are still good, especially in habitats where different mosquito species occupy the same niche at different times, which would allow for nematode recycling.
在实验室中评估了嗜蚊罗索线虫感染肯尼亚蚊虫宿主、在宿主体内发育并羽化的能力。所测试的宿主物种包括埃及伊蚊、齿斑伊蚊、多毛伊蚊、阿拉伯按蚊、库斯塔尼按蚊、致倦按蚊、冈比亚按蚊、法老按蚊、达顿库蚊、埃塞俄比亚库蚊、细纹库蚊、致倦库蚊、条纹库蚊、单带库蚊、金属色脉毛蚊、非洲曼蚊、纯色曼蚊、华丽米蚊、纯色米蚊、短须巨蚊和巴尔富氏蓝带蚊。嗜蚊罗索线虫穿透了所有测试的宿主物种，并在大多数宿主体内发育和羽化。在纯色曼蚊的所有龄期均观察到了穿透和一定程度的发育，但未观察到线虫羽化。在所有四个龄期阶段，短须巨蚊在感染嗜蚊罗索线虫后48小时内均表现出以嗜蚊罗索线虫黑化形式的抗性。在齿斑伊蚊、多毛伊蚊、达顿库蚊、条纹库蚊和华丽米蚊中观察到了线虫黑化现象，尤其是在老龄宿主中。当发生黑化时，黑化的线虫尸体在各龄期之间传递。嗜蚊罗索线虫在肯尼亚野外释放的前景仍然良好，特别是在不同蚊虫物种在不同时间占据相同生态位的栖息地，这将有利于线虫的循环利用。
