Peters Anthony, Brey Darren M, Burdick Jason A
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Tissue Eng Part B Rev. 2009 Sep;15(3):225-39. doi: 10.1089/ten.TEB.2009.0049.
As the field of tissue engineering progresses, new technology is essential to accelerate the identification of potentially translatable approaches for the repair of tissues damaged due to disease or trauma. The development of high-throughput and combinatorial technologies is helping to speed up research that is applicable to all aspects of the tissue engineering paradigm. This diverse technology can be used for both the rapid synthesis of polymers and their characterization with respect to local and bulk properties in a high-throughput fashion. The interactions of cells with many diverse materials in both two- and three-dimensions can be assessed rapidly through the use of microarrays and rapid outcome measures and with microfluidic devices for investigation of soluble factor and material combinations. Finally, small molecule screening of large libraries is being used to identify molecules that exhibit control over cell behavior, including stem cell differentiation. All of these approaches are aimed to move beyond traditional iterative methods to identify unique materials and molecules that would not have been identified otherwise. Although much of this work is only applicable for in vitro studies, future methods may translate into rapid screening of these approaches in vivo.
随着组织工程领域的不断发展，新技术对于加速识别可能适用于修复因疾病或创伤而受损组织的可转化方法至关重要。高通量和组合技术的发展有助于加快适用于组织工程范式各个方面的研究。这种多样化的技术可用于以高通量方式快速合成聚合物并对其局部和整体性质进行表征。通过使用微阵列和快速结果测量以及用于研究可溶性因子和材料组合的微流控装置，可以快速评估细胞在二维和三维空间中与多种不同材料的相互作用。最后，对大型文库进行小分子筛选，以识别能够控制细胞行为（包括干细胞分化）的分子。所有这些方法旨在超越传统的迭代方法，以识别那些否则无法识别的独特材料和分子。尽管这项工作大部分仅适用于体外研究，但未来的方法可能会转化为在体内对这些方法进行快速筛选。
