Eisenreich Wolfgang, Fischer Markus, Römisch-Margl Werner, Joshi Monika, Richter Gerald, Bacher Adelbert, Weber Stefan
Department Chemie, Technische Universität München, Garching, Germany.
Biochem Soc Trans. 2009 Apr;37(Pt 2):382-6. doi: 10.1042/BST0370382.
(13)C-NMR experiments were performed on photo-excited fully and partially (13)C-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from (13)C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN (13)C isotopologues.
对蓝光受体向光素的光激发完全和部分（13）C标记的LOV2结构域进行了（13）C核磁共振实验。在本文中，我们报道了由光诱导的蛋白质内电子转移至FMN辅因子所产生的核自旋极化色氨酸共振。结合先前从用FMN（13）C同位素类似物重构的未标记LOV2结构域的（13）C核磁共振实验获得的数据，对这些光谱进行了讨论。
