Kim Hana, Kang Keunsoo, Kim Joomyeong
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Nucleic Acids Res. 2009 May;37(9):2940-50. doi: 10.1093/nar/gkp149. Epub 2009 Mar 17.
AEBP2 is a zinc finger protein that has been shown to interact with the mammalian Polycomb Repression Complex 2 (PRC2). In the current study, we characterized this unknown protein and tested its potential targeting roles for the PRC2. AEBP2 is an evolutionarily well-conserved gene that is found in the animals ranging from flying insects to mammals. The transcription of mammalian AEBP2 is driven by two alternative promoters and produces at least two isoforms of the protein. These isoforms show developmental stage-specific expression patterns: the adult-specific larger form (51 kDa) and the embryo-specific smaller form (32 kDa). The AEBP2 protein binds to a DNA-binding motif with an unusual bipartite structure, CTT(N)15-23cagGCC with lower-case being less critical. A large fraction of AEBP2's target loci also map closely to the known target loci of the PRC2. In fact, many of these loci are co-occupied by the two proteins, AEBP2 and SUZ12. This suggests that AEBP2 is most likely a targeting protein for the mammalian PRC2 complex.
AEBP2是一种锌指蛋白，已被证明可与哺乳动物的多梳抑制复合物2（PRC2）相互作用。在本研究中，我们对这种未知蛋白进行了表征，并测试了其对PRC2的潜在靶向作用。AEBP2是一个在进化上高度保守的基因，存在于从飞虫到哺乳动物的各类动物中。哺乳动物AEBP2的转录由两个可变启动子驱动，并产生至少两种蛋白质异构体。这些异构体呈现出发育阶段特异性的表达模式：成年特异性的较大形式（51 kDa）和胚胎特异性的较小形式（32 kDa）。AEBP2蛋白与具有不寻常二分结构的DNA结合基序CTT(N)15 - 23cagGCC结合，其中小写字母部分的重要性较低。AEBP2的大部分靶位点也与PRC2的已知靶位点紧密定位。事实上，这些位点中的许多都被AEBP2和SUZ12这两种蛋白共同占据。这表明AEBP2很可能是哺乳动物PRC2复合物的一种靶向蛋白。
