Graves D A, Lang C A, Leavitt M E
Biotechnology Center, International Technology Corporation, Knoxville, TN 37923.
Appl Biochem Biotechnol. 1991 Spring;28-29:813-26. doi: 10.1007/BF02922652.
Client-funded bench-scale investigations concerning the likelihood of successfully applying biological remediation to hazardous wastes must be cost-effective, and they usually need only determine if biodegradation is likely to occur on site. To assess the potential for stimulating biodegradation, biochemical oxygen demand (BOD) was used to continuously monitor bacterial respiration during growth on mixed organic wastes from contaminated water and soil. Continuously collected oxygen-consumption data provided information on the overall metabolic activity of the resident bacterial population and permitted direct observation of the cessation of microbial respiratory activity and, thus, the termination of aerobic degradation. The correlation of biological oxygen utilization with biodegradation was confirmed using independent analytical methods. Continuous, long-term BOD analysis was applied to bench-scale studies to assess the biodegradation of mixed organic wastes from contaminated sites and industrial waste effluents. This information was used to make an initial determination regarding the need to further explore bioremediation as a potential remedial-action technology using on-site, pilot-scale testing.
由客户出资进行的关于成功应用生物修复处理危险废物可能性的实验室规模调查必须具有成本效益，而且通常只需确定现场是否可能发生生物降解。为了评估刺激生物降解的潜力，生化需氧量（BOD）被用于在以受污染水和土壤中的混合有机废物为养分生长期间持续监测细菌呼吸。持续收集的耗氧数据提供了有关常驻细菌种群总体代谢活性的信息，并允许直接观察微生物呼吸活动的停止，从而观察到好氧降解的终止。使用独立分析方法证实了生物需氧量利用与生物降解之间的相关性。持续、长期的BOD分析应用于实验室规模研究，以评估受污染场地和工业废水排放中的混合有机废物的生物降解情况。这些信息被用于初步确定是否有必要通过现场中试规模测试进一步探索生物修复作为一种潜在的补救行动技术。
