Chapatwala K D, Babu G R, Vijaya O K, Armstead E, Palumbo A V, Zhang C, Phelps T J
Division of Natural Science, Selma University, AL 36701, USA.
Appl Biochem Biotechnol. 1996 Spring;57-58:827-35. doi: 10.1007/BF02941762.
As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer was used to measure the rate of carbon dioxide (CO2) production during the respiration of the sediment samples. The rate of respiration of the sediment samples ranged from 0.035-0.6 microL CO2/h/g of the sediment. The sediment samples showing maximum (0.6 microL CO2/h/g) and minimum (0.035 microL CO2/h/g) production of CO2 were selected to study the effect of micronutrient-yeast extract (0.5 and 1.0 micrograms/g of the sediment) and water (0.5 and 1.0 mL) on the rate of CO2 production. The rate of CO2 production increased with the addition of water, but increased approx 2 orders of magnitude (from 0.26 to an average of 23.5 microL CO2/h/g) when 1.0 g/g yeast extract was added to the sediment samples. In these coastal sediments, temperature, depth, and addition of water influenced microbial activity, but the addition of 1.0 microgram/g yeast extract as a micronutrient rapidly increased the rate of CO2 production 2 orders of magnitude.
作为我们对地下环境中微生物异质性研究的一部分，我们检测了从弗吉尼亚州牡蛎市附近一个沿海地点采集的沉积物样本的微生物呼吸作用。该地点的沉积物为未固结的细至粗沙滩沙和砾石。使用哥伦布仪器公司的微量氧最大值呼吸仪来测量沉积物样本呼吸过程中二氧化碳（CO₂）的产生速率。沉积物样本的呼吸速率范围为0.035 - 0.6微升CO₂/小时/克沉积物。选择显示CO₂产生量最大（0.6微升CO₂/小时/克）和最小（0.035微升CO₂/小时/克）的沉积物样本，以研究微量营养素 - 酵母提取物（0.5和1.0微克/克沉积物）和水（0.5和1.0毫升）对CO₂产生速率的影响。CO₂产生速率随水的添加而增加，但当向沉积物样本中添加1.0克/克酵母提取物时，增加了约2个数量级（从0.26增加到平均23.5微升CO₂/小时/克）。在这些沿海沉积物中，温度、深度和水的添加影响微生物活性，但添加1.0微克/克酵母提取物作为微量营养素会使CO₂产生速率迅速增加2个数量级。
