Celier C, Cresteil T
INSERM U75, Paris, France.
Arch Biochem Biophys. 1991 Nov 1;290(2):407-10. doi: 10.1016/0003-9861(91)90559-2.
The absence of changes in the overall hepatic cytochrome P450 content after administration of 3-methylcholanthrene (MC) to congenitally jaundiced Gunn rats is believed to be related to a limited heme availability in this strain of rat. The amount of available heme, estimated by tryptophan pyrrolase activity, shows a substantial decrease in control Gunn versus control Wistar rats. This reduction is moderately enhanced by MC treatment in Gunn rats but is abolished after phenobarbital administration. Heme oxygenase activity is diminished in Gunn rats and consequently is not responsible for the decrease in the hepatic heme availability. These data point out that the depletion of the intracellular heme can lead to a limitation in the synthesis of cytochrome P450 isoenzymes in the MC-induced Gunn rat.
给先天性黄疸的冈恩大鼠注射3-甲基胆蒽（MC）后，肝脏中细胞色素P450总量未发生变化，这被认为与该品系大鼠血红素可用性有限有关。通过色氨酸吡咯酶活性估算的可用血红素量显示，对照冈恩大鼠与对照Wistar大鼠相比大幅减少。在冈恩大鼠中，MC处理适度增强了这种减少，但在给予苯巴比妥后这种减少被消除。血红素加氧酶活性在冈恩大鼠中降低，因此并非肝脏血红素可用性降低的原因。这些数据表明，细胞内血红素的消耗会导致MC诱导的冈恩大鼠中细胞色素P450同工酶合成受限。
