Tefft P M, Bone L W
J Nematol. 1985 Jul;17(3):275-9.
Root diffusate from soybean plants caused greater hatching of Heterodera glycines eggs during vegetative growth of the host, but the activity declined with plant senescence. Chelation of the root diffusate with ethylenediamine tetraacetic acid (EDTA) significantly increased hatching activity for H. glycines eggs. Diffusate from leafless plants caused little hatching, whereas treatment of intact plants with the growth regulators gibberellin and kinetin had no effect on the hatching activity of root diffusate. Treating H. glycines eggs with zinc chloride and root diffusate reduced egg hatching from zinc chloride alone. Levels of zinc in the root diffusate were insufficient to induce egg hatch, based on analysis by atomic absorption spectrophotometry. The enzymatic activity of leucine aminopeptidase in H. glycines eggs was not altered by treatment with chelated or nonchelated root diffusate.
在宿主营养生长阶段，大豆植株的根系渗出物可使大豆胞囊线虫卵的孵化率显著提高，但随着植株衰老，这种活性会下降。用乙二胺四乙酸（EDTA）螯合根系渗出物可显著提高大豆胞囊线虫卵的孵化活性。无叶植株的渗出物几乎不能引起孵化，而用生长调节剂赤霉素和激动素处理完整植株对根系渗出物的孵化活性没有影响。用氯化锌和根系渗出物处理大豆胞囊线虫卵，可降低单独使用氯化锌时的卵孵化率。基于原子吸收分光光度法分析，根系渗出物中的锌含量不足以诱导卵孵化。用螯合或未螯合的根系渗出物处理后，大豆胞囊线虫卵中的亮氨酸氨肽酶的酶活性没有改变。
