Department of Physiology, Southern Illinois University, 62901, Carbondale, Illinois.
J Chem Ecol. 1984 Feb;10(2):361-72. doi: 10.1007/BF00987863.
Egg hatching of the soybean cyst nematode,Heterodera glycines, was not affected by millimolar concentrations of calcium sulfate or calcium chloride. However, zinc chloride and zinc sulfate caused strong and moderate increases in hatching, respectively. The inhibitors of calcium transport, ruthenium red and lanthanum chloride, and calcium ionophore A23187 had no effect on hatching in the presence or absence of 3 mM zinc chloride. Selected chelators decreased the zinc-induced hatching ofH. glycines eggs. Eggs exhibited a formation constant with zinc between 5.5 and 11.2. The addition of zinc chloride after chelation with EDTA and rinsing caused expected hatching rates. Concentrations of calcium chloride, manganese chloride, and magnesium chloride had no effect on hatching of eggs in zinc chloride, but reduced hatching at higher concentrations, possibly by osmotic influences. Hatching of eggs was increased as the time of exposure to zinc chloride was increased and was maximal at 28 °C and a pH of 5.3. Picrolonic acid, a known hatching stimulant, increasedH. glycines hatching, while sodium metavanadate had no effect. Analysis of seasonal hatching during 1981-1982 in untreated control eggs indicated that hatching was most pronounced in May.
大豆胞囊线虫（Heterodera glycines）的卵孵化不受硫酸钙或氯化钙的毫摩尔浓度的影响。然而，氯化锌和硫酸锌分别导致强烈和适度的孵化增加。钙转运抑制剂钌红和氯化镧，以及钙载体 A23187，在存在或不存在 3mM 氯化锌的情况下对孵化均无影响。选定的螯合剂降低了锌诱导的大豆胞囊线虫卵孵化。卵对锌的形成常数在 5.5 到 11.2 之间。用 EDTA 螯合并冲洗后添加氯化锌会导致预期的孵化率。氯化钙、氯化锰和氯化镁的浓度对氯化锌中的卵孵化没有影响，但在较高浓度下会降低孵化率，可能是渗透压的影响。随着暴露于氯化锌的时间增加，卵的孵化增加，在 28°C 和 pH5.3 时达到最大值。已知的孵化刺激物皮考啉酸增加了大豆胞囊线虫的孵化，而偏钒酸钠则没有影响。对 1981-1982 年未经处理的对照卵进行的季节性孵化分析表明，孵化在 5 月最为明显。
