Stender C, Glazer I, Orion D
J Nematol. 1986 Jan;18(1):37-43.
Hydroxyurea (HU) at concentrations of 10 or 20 mg/liter was included in a medium on which excised tomato roots infected with the root-knot nematode Meloidogyne javanica were grown. In the HU, treated roots, giant cells were small and contained large vacuoles. Giant cell nuclei were amoeboidal with relatively small nucleoli in treated roots, compared with giant cells of nontreated galls. In treated-root giant cells, the cytoplasm was diffuse and few organelles such as mitochondria, dictyosomes, and endoplasmic reticulum were detected; also, walls of giant cells were thin with less extensive ingrowths than in nontreated roots. We conclude that HU suppressed normal giant cell formation interfering with its function as a feeding cell.
将浓度为10或20毫克/升的羟基脲（HU）添加到一种培养基中，在此培养基上培养感染了爪哇根结线虫的番茄离体根。在HU处理过的根中，巨型细胞较小且含有大液泡。与未处理虫瘿的巨型细胞相比，处理过的根中巨型细胞核呈变形虫状，核仁相对较小。在处理过的根的巨型细胞中，细胞质呈弥散状，检测到的线粒体、高尔基体和内质网等细胞器较少；此外，巨型细胞的细胞壁较薄，向内生长的程度不如未处理的根。我们得出结论，HU抑制了正常巨型细胞的形成，干扰了其作为取食细胞的功能。
