壳寡糖对链脲佐菌素诱导的糖尿病大鼠胰岛细胞的抗氧化活性
Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats.
作者信息
Yuan Wen-Peng, Liu Bing, Liu Chang-Heng, Wang Xiao-Jun, Zhang Mian-Song, Meng Xiu-Mei, Xia Xue-Kui
机构信息
Biology Institute of Shandong Academy of Sciences, Jinan 250014, Shandong Province, China.
出版信息
World J Gastroenterol. 2009 Mar 21;15(11):1339-45. doi: 10.3748/wjg.15.1339.
Abstract
AIM
To investigate the antioxidant activity of chito-oligosaccharides (COSs) on pancreatic islet cells in diabetic rats induced by streptozotocin.
METHODS
The antioxidant effect of COSs on pancreatic islet cells was detected under optical microscopy and with colorimetric assay and gel electrophoresis. The activities of glutathione peroxidase and superoxide dismutase, total antioxidant capacity, and content of malondialdehyde in serum and tissue slices of pancreas were examined after 60 d to determine the effect of COSs in streptozotocin-induced diabetes in rats.
RESULTS
COSs can prohibit the apoptosis of pancreatic islet cells. All concentrations of COSs can improve the capability of total antioxidant capacity and activity of superoxide dismutase and decrease the content of malondialdehyde drastically. Morphological investigation in the pancreas showed that COSs have resulted in the reduction of islets, loss of pancreatic cells, and nuclear pyknosis of pancreatic cells.
CONCLUSION
COSs possess various biological activities and can be used in the treatment of diabetes mellitus.
摘要
目的
研究壳寡糖（COSs）对链脲佐菌素诱导的糖尿病大鼠胰岛细胞的抗氧化活性。
方法
在光学显微镜下，采用比色法和凝胶电泳检测COSs对胰岛细胞的抗氧化作用。60天后检测血清和胰腺组织切片中谷胱甘肽过氧化物酶和超氧化物歧化酶的活性、总抗氧化能力以及丙二醛含量，以确定COSs对链脲佐菌素诱导的大鼠糖尿病的影响。
结果
COSs可抑制胰岛细胞凋亡。所有浓度的COSs均可提高总抗氧化能力和超氧化物歧化酶活性，并显著降低丙二醛含量。胰腺形态学研究表明，COSs导致胰岛减少、胰腺细胞丢失和胰腺细胞核固缩。
结论
COSs具有多种生物学活性，可用于治疗糖尿病。
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