利用电子自旋共振光谱法测定壳寡糖的自由基清除活性。

Free radical scavenging activity of chitooligosaccharides by electron spin resonance spectrometry.

作者信息

Park Pyo-Jam, Je Jae-Young, Kim Se-Kwon

机构信息

Department of Chemistry, Pukyong National University, Busan 608-737, Korea.

出版信息

J Agric Food Chem. 2003 Jul 30;51(16):4624-7. doi: 10.1021/jf034039+.

DOI:10.1021/jf034039+

PMID:14705887

Abstract

The radical scavenging effects of chitooligosaccharides (COSs) on hydroxyl radical, superoxide radical, alkyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were investigated using a spin-trapping electron spin resonance (ESR) method and compared with the ESR signal intensity. COSs exhibited strong scavenging activity on hydroxyl radical and superoxide radical and weak scavenging activity on alkyl radical and DPPH radical. The radical scavenging activity of COSs increased with increment of concentration, and it was also dependent on molecular weight. These results suggest that the scavenging activity of COSs is dependent on their molecular weights and tested radicals.

摘要

采用自旋捕集电子自旋共振（ESR）方法研究了壳寡糖（COSs）对羟基自由基、超氧自由基、烷基自由基和1,1-二苯基-2-苦基肼（DPPH）自由基的清除作用，并与ESR信号强度进行了比较。COSs对羟基自由基和超氧自由基表现出较强的清除活性，对烷基自由基和DPPH自由基表现出较弱的清除活性。COSs的自由基清除活性随浓度增加而增强，且还取决于分子量。这些结果表明，COSs的清除活性取决于其分子量和所测试的自由基。