Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Science, Sichuan University, 610064, Chengdu, People's Republic of China.
Mol Biol Rep. 2010 Feb;37(2):661-8. doi: 10.1007/s11033-009-9510-x. Epub 2009 Mar 19.
A full-length cDNA of Rubisco activase (IBrcaI) was cloned from sweet potato (Ipomoea batatas (L.) Lam) using Rapid-Amplification of cDNA Ends (RACE). IBrcaI contains a 1,347 bp open reading frame encoding a protein of 439 amino acids. The sequence alignment of multiple Rubisco activase genes from sweet potato and other plants showed high homology at two previously described ATP-binding sites. Western blot analysis indicated that there are two Rubisco activase proteins in sweet potato. Expression of IBrcaI was only detected in leaves. In the 14 h light and 10 h dark photoperiods, maximal and minimal IBrcaI mRNA expression levels were detected at 8:00 in the morning and at midnight, respectively.
采用快速扩增 cDNA 末端（RACE）技术，从甘薯（Ipomoea batatas (L.) Lam）中克隆得到全长 Rubisco 激活酶（IBrcaI）cDNA。IBrcaI 含有一个 1347bp 的开放阅读框，编码 439 个氨基酸的蛋白质。甘薯和其他植物的多个 Rubisco 激活酶基因的序列比对显示，在两个先前描述的 ATP 结合位点具有高度同源性。Western blot 分析表明甘薯中有两种 Rubisco 激活酶蛋白。IBrcaI 的表达仅在叶片中检测到。在 14 h 光照和 10 h 黑暗的光周期下，IBrcaI mRNA 的最大和最小表达水平分别在早上 8 点和午夜达到。
