Wei Yu-zhe, Li Chun-feng, Xue Ying-wei
Department of Gastrointestinal Surgery, The Tumor Hospital, Harbin Medical University, Harbin 150081, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2009 Mar;12(2):145-9.
To investigate the expression of transcription factor SP1, vascular endothelial growth factor(VEGF) and CD34 in serosa-infiltrating gastric cancer and their relationship with biological behavior and survival rate.
Immunohistochemical technique was used to detect the expression of SP1, VEGF and CD34(described by microvessel density, MVD) in 68 specimens with serosa-infiltrating gastric cancer.
The positive expression rates of SP1 and VEGF in serosa-infiltrating gastric cancer were 50.0% and 52.9% respectively. In positive SP1 specimens, the positive rate of VEGF(73.5%) was significantly higher than that of negative SP1 specimens (32.4%, chi(2)=11.57, P=0.01). The mean tumor MVD was correlated with the expression levels of SP1 and VEGF(P<0.01). There was a significant correlation of the SP1 expression with tumor size and growth pattern(P =0.01). The expression levels of VEGF and MVD were correlated with Borrmann types, cell differentiation, metastatic lymph nodes and growth pattern(P<0.01). Univariate analysis revealed that SP1 and VEGF expression, MVD, Borrmann types, lymph node metastasis, tumor size and growth pattern were significant prognostic factors related to survival time. Multivariate analysis showed that SP1 expression, MVD and growth pattern were independently prognostic factors of poor survival.
The activation of SP1 contributes to angiogenesis and metastasis in gastric cancer through the up-regulation of VEGF. SP1, VEGF and MVD may serve as valuable indicators of biological behavior of gastric cancer. SP1 protein expression is not related with the number of metastatic lymph nodes. SP1 expression and MVD may serve as valuable indicators of prognosis in gastric carcinoma.
探讨转录因子SP1、血管内皮生长因子（VEGF）及CD34在浆膜浸润性胃癌中的表达及其与生物学行为和生存率的关系。
采用免疫组织化学技术检测68例浆膜浸润性胃癌标本中SP1、VEGF的表达及CD34（以微血管密度，MVD表示）。
浆膜浸润性胃癌中SP1和VEGF的阳性表达率分别为50.0%和52.9%。在SP1阳性标本中，VEGF的阳性率（73.5%）显著高于SP1阴性标本（32.4%，χ²=11.57，P=0.01）。肿瘤平均MVD与SP1和VEGF的表达水平相关（P<0.01）。SP1表达与肿瘤大小和生长方式显著相关（P=0.01）。VEGF表达水平和MVD与Borrmann分型、细胞分化、淋巴结转移及生长方式相关（P<0.01）。单因素分析显示，SP1和VEGF表达、MVD、Borrmann分型、淋巴结转移、肿瘤大小及生长方式是与生存时间相关的重要预后因素。多因素分析表明，SP1表达、MVD及生长方式是生存不良的独立预后因素。
SP1的激活通过上调VEGF促进胃癌血管生成和转移。SP1、VEGF和MVD可能是胃癌生物学行为的有价值指标。SP1蛋白表达与淋巴结转移数目无关。SP1表达和MVD可能是胃癌预后的有价值指标。
