Nesvaderani Maryam, Matsumoto Izuru, Sivagnanasundaram Sinthuja
Discipline of Pathology, University of Sydney, New South Wales, Australia.
Aust N Z J Psychiatry. 2009 Apr;43(4):310-22. doi: 10.1080/00048670902721103.
The purpose of the present study was to identify differentially expressed proteins in the anterior and posterior hippocampus of brains of schizophrenia patients compared to neurologically healthy controls.
Proteins extracted from fresh frozen post-mortem posterior and anterior hippocampus for nine schizophrenia and nine control individuals, and seven schizophrenia and seven control individuals, respectively, were screened for differential expression using 2-D gel electrophoresis and mass spectrometry.
A significantly larger number of protein spots were differentially expressed in the anterior (n = 43) compared to the posterior (n = 16) hippocampus, representing 34 and 14 unique proteins, respectively. These proteins are involved in cytoskeleton structure and function, neurotransmission and mitochondrial function.
Based on the aberrant protein expression profiles, the anterior hippocampus appears to be more involved in schizophrenia pathogenesis than the posterior hippocampus. Furthermore, consistent with previous findings, we found molecular evidence to support abnormal neuronal cytoarchitecture and function, neurotransmission and mitochondrial function in the schizophrenia hippocampus.
本研究旨在确定与神经功能正常的对照者相比，精神分裂症患者大脑前、后海马体中差异表达的蛋白质。
分别从9例精神分裂症患者和9例对照个体、7例精神分裂症患者和7例对照个体的新鲜冷冻尸检后海马体和前海马体中提取蛋白质，使用二维凝胶电泳和质谱法筛选差异表达。
与后海马体（n = 16）相比，前海马体（n = 43）中差异表达的蛋白斑点数量明显更多，分别代表34种和14种独特蛋白质。这些蛋白质参与细胞骨架结构和功能、神经传递及线粒体功能。
基于异常的蛋白质表达谱，前海马体似乎比后海马体更参与精神分裂症的发病机制。此外，与先前的研究结果一致，我们发现分子证据支持精神分裂症海马体中神经元细胞结构和功能、神经传递及线粒体功能异常。
