Bhogal Nirmal, Jalali Farid, Bristow Robert G
Applied Molecular Oncology and Radiation Medicine Program, Ontario Cancer Institute/Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario, Canada.
Int J Radiat Biol. 2009 Sep;85(9):732-46. doi: 10.1080/09553000902785791.
It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as gamma-H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy in vitro and in vivo in measuring intranuclear foci following irradiation.
Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.
通过使用免疫荧光显微镜和数字图像分析来测量DNA修复灶（如γ-H2AX）的诱导和消退，现在已能够在组织中检测DNA双链断裂（DSB）。本综述将重点介绍组织显微镜检查和分析的主要工具和方法。还将讨论在体外和体内使用显微镜测量辐射后核内灶的实际注意事项。
基于计算机的图像分析算法允许使用3D共聚焦图像对灶和蛋白质-蛋白质相互作用进行客观和定量分析。最后，我们回顾了将DNA修复灶作为正常组织中生物剂量计或DNA修复生物标志物进行研究的文献。
