Jayasinghe Ruchira, Hai Niu Lin, Coram Tristan E, Kong Stephan, Kaganovitch Janna, Xue Charlie C L, Li Chun G, Pang Edwin C K
Division of Chinese Medicine, School of Health Sciences, RMIT University, Bundoora, VIC, Australia.
Planta Med. 2009 Aug;75(10):1180-5. doi: 10.1055/s-0029-1185484. Epub 2009 Mar 18.
The accurate identification of medicinal plants is becoming increasingly important due to reported concerns about purity, quality and safety. The previously developed prototype subtracted diversity array (SDA) had been validated for the ability to distinguish clade-level targets in a phylogenetically accurate manner. This study represents the rigorous investigation of the SDA for genotyping capabilities, including the genotyping of plant species not included during the construction of the SDA, as well as to lower classification levels including family and species. The results show that the SDA, in its current form, has the ability to accurately genotype species not included during SDA development to clade level. Additionally, for those species that were included during SDA development, genotyping is successful to the family level, and to the species level with minor exceptions. Twenty polymorphic SDA features were sequenced in a first attempt to characterize the polymorphic DNA between species, which showed that transposon-like sequences may be valuable as polymorphic features to differentiate angiosperm families and species. Future refinements of the SDA to allow more sensitive genotyping are discussed with the overall goal of accurate medicinal plant identification in mind.
由于对纯度、质量和安全性的担忧日益增加，药用植物的准确鉴定变得越来越重要。先前开发的原型减法多样性阵列（SDA）已被验证具有以系统发育准确的方式区分进化枝水平目标的能力。本研究代表了对SDA基因分型能力的严格调查，包括对在构建SDA时未包括的植物物种进行基因分型，以及对包括科和种在内的较低分类水平进行基因分型。结果表明，当前形式的SDA能够将在SDA开发过程中未包括的物种准确基因分型到进化枝水平。此外，对于那些在SDA开发过程中包括的物种，基因分型在科级水平上是成功的，在种级水平上除了少数例外也是成功的。首次对20个多态性SDA特征进行了测序，以表征物种间的多态性DNA，结果表明转座子样序列作为区分被子植物科和种的多态性特征可能很有价值。讨论了SDA未来的改进，以实现更灵敏的基因分型，总体目标是准确鉴定药用植物。
