线虫秀丽新小杆线虫中显性滚动突变体的分子克隆及DNA介导转化的建立。
Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus.
作者信息
Schlager Benjamin, Wang Xiaoyue, Braach Georg, Sommer Ralf J
机构信息
Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, Tübingen, Germany.
出版信息
Genesis. 2009 May;47(5):300-4. doi: 10.1002/dvg.20499.
PMID:19298013
Abstract
We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus.
摘要
我们报道了太平洋小杆线虫（Pristionchus pacificus）一个显性Roller突变体的分子克隆，该突变体编码一种表皮胶原蛋白。我们利用该突变位点作为标记，通过构建复杂阵列来开发转基因技术，并展示了基于荧光蛋白的太平洋小杆线虫转录报告构建体。
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