Eren Güniz Göze, Roca Marianne, Han Ziduan, Lightfoot James W
Max Planck Research Group Genetics of Behavior, Max Planck Institute for Neurobiology of Behavior - caesar, Bonn, Germany.
Department for Integrative Evolutionary Biology, Max Planck Institute for Biology, Tuebingen, Germany.
MicroPubl Biol. 2022 May 29;2022. doi: 10.17912/micropub.biology.000576. eCollection 2022.
Transgenes are widely used throughout molecular biology for numerous applications. In stable transgenes are usually generated by microinjection into the germline establishing extrachromosomal arrays. Furthermore, numerous technologies exist to integrate transgenes into the genome. In the nematode transgenes are possible, however, their establishment is less efficient and dependent on the formation of complex arrays containing the transgene of interest and host carrier DNA. Additionally, genomic integration has only been reported via biolistic methods. Here we describe a simple technique using UV irradiation to facilitate the integration of transgenes into the genome.
转基因在整个分子生物学中被广泛应用于众多领域。在稳定转基因中，通常通过显微注射到生殖系中来产生，从而建立染色体外阵列。此外，存在多种将转基因整合到基因组中的技术。在线虫中，转基因是可行的，然而，其建立效率较低，并且依赖于包含目标转基因和宿主载体DNA的复杂阵列的形成。此外，仅通过生物弹道方法报道过基因组整合。在此，我们描述了一种使用紫外线照射来促进转基因整合到线虫基因组中的简单技术。
