Nakamoto Masatoshi, Muramatsu Shinsuke, Yoshida Shoko, Matsuda Masaru, Nagahama Yoshitaka, Shibata Naoki
Department of Biology, Shinshu University, Matsumoto, Nagano, Japan.
Genesis. 2009 May;47(5):289-99. doi: 10.1002/dvg.20498.
Oryzias luzonensis is closely related to the medaka, O. latipes. The sex of both species is determined by an XX-XY system. However, the testis determining gene (DMY/Dmrt1bY) found in O. latipes does not exist in O. luzonensis. Instead, a different gene is thought to act as a testis determining gene. In this study, we focused the gonadal sex differentiation process in O. luzonensis under different testis determining gene. First, we observed the gonadal development of O. luzonensis histologically. We then analyzed the expression of Sox9a2/Sox9b, Dmrt1, and Foxl2 during early development. Our results suggest that the sexual differentiation of germ cells in O. luzonensis is initiated later than in O. latipes. However, the timing of the sexual differentiation of the supporting cell linage is similar between the species.
吕宋青鳉与青鳉（O. latipes）亲缘关系密切。这两个物种的性别均由XX-XY系统决定。然而，在青鳉中发现的睾丸决定基因（DMY/Dmrt1bY）在吕宋青鳉中并不存在。相反，一种不同的基因被认为起到睾丸决定基因的作用。在本研究中，我们聚焦于不同睾丸决定基因作用下吕宋青鳉的性腺性别分化过程。首先，我们从组织学角度观察了吕宋青鳉的性腺发育。然后，我们分析了早期发育过程中Sox9a2/Sox9b、Dmrt1和Foxl2的表达情况。我们的结果表明，吕宋青鳉生殖细胞的性别分化比青鳉启动得更晚。然而，这两个物种中支持细胞系的性别分化时间相似。
