Diep Cao Ngoc, Cam Pham My, Vung Nguyen Hoai, Lai To Thi, My Nguyen Thi Xuan
Microbiology Department, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam.
Bioresour Technol. 2009 Aug;100(16):3787-91. doi: 10.1016/j.biortech.2009.02.021. Epub 2009 Mar 18.
The aim of this study was to explore the potential for reducing soluble N load in fishpond wastewater using naturally occurring denitrifying bacteria. Twenty-seven isolates were selected from in wastewater (liquid/solid) of catfish-ponds located along the Tien river, in the Mekong Delta, Vietnam in SW-LB medium (artificial seawater Luria-Britani medium) supplemented with 10 mM NH4 and NO3 and twenty-five isolates were identified as Pseudomonas stutzeri based on similarity of PCR-16S rRNA using universal primers and specific primers. Four isolates were effective in lowering soluble N (NH4, NO2 and NO3) levels in fishpond water from 10 mg/L to negligible amounts after four days. Further experiments are underway to determine the fate of N lost from solution and the relative activity of ammonia oxidation, and nitrite and nitrate reduction by P. stutzeri isolates.
本研究的目的是探索利用天然存在的反硝化细菌降低鱼塘废水中可溶性氮负荷的潜力。从越南湄公河三角洲天江沿岸鲶鱼塘的废水（液体/固体）中，在添加了10 mM NH4和NO3的SW-LB培养基（人工海水鲁氏培养基）中筛选出27株菌株，基于通用引物和特异性引物的PCR-16S rRNA相似性，将其中25株鉴定为施氏假单胞菌。四天后，有四株菌株能有效将鱼塘水中的可溶性氮（NH4、NO2和NO3）水平从10 mg/L降至可忽略不计的量。目前正在进行进一步实验，以确定溶液中氮的去向以及施氏假单胞菌分离株的氨氧化、亚硝酸盐和硝酸盐还原的相对活性。
