Ponce M Lourdes
National Eye Institute, Bethesda, Maryland, USA.
Methods Mol Biol. 2009;467:183-8. doi: 10.1007/978-1-59745-241-0_10.
Neovascularization plays a role in several pathological conditions, including tumor growth, arthritis, and choroidal neovascularization. Investigators from different fields can choose from several available angiogenesis assays according to their specific needs. This chapter describes an easy-to-perform assay that is based on the differentiation of endothelial cells and the formation of tube-like structures on an extracellular matrix, Matrigel. The assay can be used to screen compounds for angiogenic activity or to determine if it has an effect on angiogenesis, depending on the conditions chosen. It is a quick assay, easy to set up, and highly reproducible. It can be used to test one or two samples, or it can quickly be scaled up to screen hundreds of compounds. The flexibility that this assay provides makes it a good first choice to test if a compound or a series of compounds may play a role in angiogenesis.
新生血管形成在多种病理状况中发挥作用，包括肿瘤生长、关节炎和脉络膜新生血管形成。来自不同领域的研究人员可根据其特定需求，从几种现有的血管生成检测方法中进行选择。本章描述了一种易于操作的检测方法，该方法基于内皮细胞的分化以及在细胞外基质基质胶上形成管状结构。根据所选条件，该检测方法可用于筛选具有血管生成活性的化合物，或确定其是否对血管生成有影响。这是一种快速检测方法，易于设置且具有高度可重复性。它可用于测试一个或两个样本，也可迅速扩大规模以筛选数百种化合物。该检测方法所提供的灵活性使其成为测试一种化合物或一系列化合物是否可能在血管生成中起作用的良好首选方法。
