Melhem R F, Strahler J R, Hailat N, Zhu X X, Hanash S M
Department of Pediatrics, University of Michigan Medical School, Ann Arbor 48109-0510.
Biochem Biophys Res Commun. 1991 Sep 30;179(3):1649-55. doi: 10.1016/0006-291x(91)91764-4.
Op18 is a highly conserved major cytosolic phosphoprotein that is expressed at high levels in acute leukemia and in neuroblastoma. In this study we present evidence pointing to a role for Op18 in cellular proliferation. Blocking of Op18 mRNA translation using antisense oligonucleotides delayed entrance of mitotically stimulated normal peripheral blood lymphocytes into the S phase. Moreover treatment of HL-60 promyelocytic leukemia cells with DMSO or PMA which induced terminal differentiation resulted in a decrease in the level of Op18 RNA and protein. Inhibition of lymphoid proliferation with cyclosporin also resulted in reduced Op18 levels.
Op18是一种高度保守的主要胞质磷蛋白，在急性白血病和神经母细胞瘤中高水平表达。在本研究中，我们提供的证据表明Op18在细胞增殖中发挥作用。使用反义寡核苷酸阻断Op18 mRNA翻译会延迟有丝分裂刺激的正常外周血淋巴细胞进入S期。此外，用二甲基亚砜（DMSO）或佛波酯（PMA）处理HL-60早幼粒细胞白血病细胞诱导其终末分化，导致Op18 RNA和蛋白质水平降低。用环孢素抑制淋巴细胞增殖也会导致Op18水平降低。
