Centre for Nutrition and Food Sciences, The University of Queensland, Brisbane, Qld, Australia.
J Appl Microbiol. 2009 Aug;107(2):576-83. doi: 10.1111/j.1365-2672.2009.04226.x. Epub 2009 Mar 16.
To determine the effect of carbon sources on cellulose produced by Gluconacetobacter xylinus strain ATCC 53524, and to characterize the purity and structural features of the cellulose produced.
Modified Hestrin Schramm medium containing the carbon sources mannitol, glucose, glycerol, fructose, sucrose or galactose were inoculated with Ga. xylinus strain ATCC 53524. Plate counts indicated that all carbon sources supported growth of the strain. Sucrose and glycerol gave the highest cellulose yields of 3.83 and 3.75 g l(-1) respectively after 96 h fermentation, primarily due to a surge in cellulose production in the last 12 h. Mannitol, fructose or glucose resulted in consistent rates of cellulose production and yields of >2.5 g l(-1). Solid state (13)C CP/MAS NMR revealed that irrespective of the carbon source, the cellulose produced by ATCC 53524 was pure and highly crystalline. Scanning electron micrographs illustrated the densely packed network of cellulose fibres within the pellicles and that the different carbon sources did not markedly alter the micro-architecture of the resulting cellulose pellicles.
The production rate of bacterial cellulose by Ga. xylinus (ATCC 53524) was influenced by different carbon sources, but the product formed was indistinguishable in molecular and microscopic features.
Our studies for the first time examined the influence of different carbon sources on the rate of cellulose production by Ga. xylinus ATCC 53524, and the molecular and microscopic features of the cellulose produced.
确定碳源对木醋酸杆菌（Gluconacetobacter xylinus）菌株 ATCC 53524 所产纤维素的影响，并对所产纤维素的纯度和结构特征进行表征。
用改良的 Hestrin-Schramm 培养基接种木醋酸杆菌 ATCC 53524，培养基中含有甘露醇、葡萄糖、甘油、果糖、蔗糖或半乳糖等碳源。平板计数表明，所有碳源均支持该菌株的生长。蔗糖和甘油发酵 96 h 后分别得到 3.83 和 3.75 g/L 的最高纤维素产量，主要是由于在最后 12 h 纤维素产量激增。甘露醇、果糖或葡萄糖导致纤维素的产率始终保持在 2.5 g/L 以上。固态（13）C CP/MAS NMR 表明，无论碳源如何，ATCC 53524 所产纤维素均为纯品且结晶度高。扫描电子显微镜图说明了在菌膜内纤维素纤维的密集网络，并且不同的碳源没有明显改变所得纤维素菌膜的微观结构。
不同碳源影响木醋酸杆菌（ATCC 53524）产细菌纤维素的速率，但形成的产物在分子和微观特征上没有明显差异。
本研究首次考察了不同碳源对木醋酸杆菌 ATCC 53524 纤维素产率的影响，以及所产纤维素的分子和微观特征。
