Skaradziński Grzegorz, Janek Tomasz, Śliwka Paulina, Skaradzińska Aneta, Łaba Wojciech
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, Poland.
Int J Mol Sci. 2025 Jun 24;26(13):6059. doi: 10.3390/ijms26136059.
Bacterial cellulose (BC), an extracellular polysaccharide synthesized by various bacterial strains. It exhibits high tensile strength, water retention, crystallinity, and biocompatibility, making it valuable in biomedical, cosmetic, food, textile, and paper industries. This study examined the effects of six carbon sources on BC production by , identifying fructose as the most effective. A Box-Behnken experimental design was employed to investigate the effects of three variables (fructose concentration, temperature, and cultivation time) on cellulose yield. The optimized cultivation conditions were: fructose concentration of 227.5 g/L, temperature of 28.0 °C, and cultivation time of 295 h, resulting in a BC yield of 63.07 ± 2.91 g/L. Subsequently, BC's potential as a bacteriophage carrier was assessed. phage T4 and phage vB_SauS_CS1 (CS1) were immobilized within BC hydrogels, and their antibacterial activities were assessed through in vitro experiments. These findings suggest BC's promise as a phage delivery platform for biomedical applications.
细菌纤维素（BC）是由多种细菌菌株合成的一种细胞外多糖。它具有高拉伸强度、保水性、结晶度和生物相容性，这使其在生物医学、化妆品、食品、纺织和造纸工业中具有重要价值。本研究考察了六种碳源对[具体细菌名称]生产BC的影响，确定果糖是最有效的碳源。采用Box-Behnken实验设计来研究三个变量（果糖浓度、温度和培养时间）对纤维素产量的影响。优化的培养条件为：果糖浓度227.5 g/L、温度28.0℃、培养时间295 h，BC产量为63.07±2.91 g/L。随后，评估了BC作为噬菌体载体的潜力。将噬菌体T4和噬菌体vB_SauS_CS1（CS1）固定在BC水凝胶中，并通过体外实验评估它们的抗菌活性。这些发现表明BC作为生物医学应用的噬菌体递送平台具有前景。
