Nguyen Vu Tuan, Flanagan Bernadine, Gidley Michael J, Dykes Gary A
School of Land, Crop and Food Sciences, University of Queensland, St Lucia, Queensland, Australia.
Curr Microbiol. 2008 Nov;57(5):449-53. doi: 10.1007/s00284-008-9228-3. Epub 2008 Aug 14.
The aims of this work were to characterize and improve cellulose production by a Gluconoacetobacter xylinus strain isolated from Kombucha and determine the purity and some structural features of the cellulose from this strain. Cellulose yield in tea medium with both black tea and green tea and in Hestrin and Schramm (HS) medium under both static and agitated cultures was compared. In the tea medium, the highest cellulose yield was obtained with green tea (approximately 0.20 g/L) rather than black tea (approximately 0.14 g/L). Yield in HS was higher (approximately 0.28 g/L) but did not differ between static and agitated incubation. (1)H-NMR and (13)C-NMR spectroscopy indicated that the cellulose is pure (free of acetan) and has high crystallinity, respectively. Cellulose yield was improved by changing the type and level of carbon and nitrogen source in the HS medium. A high yield of approximately 2.64 g/L was obtained with mannitol at 20 g/L and corn steep liquor at 40 g/L in combination. In the tea medium, tea at a level of 3 g/L gave the highest cellulose yield and the addition of 3 g/L of tea to the HS medium increased cellulose yield to 3.34 g/L. In conclusion, the G. xylinus strain from Kombucha had different cellulose-producing characteristics than previous strains isolated from fruit. Cellulose was produced in a pure form and showed high potential applicability. Our studies extensively characterized cellulose production from a G. xylinus strain from Kombucha for the first time, indicating both similarities and differences to strains from different sources.
本研究的目的是对从康普茶中分离出的木醋杆菌菌株生产纤维素的特性进行表征并加以改进，并确定该菌株所产纤维素的纯度和一些结构特征。比较了在红茶和绿茶茶培养基以及赫斯特林和施拉姆（HS）培养基中，该菌株在静置和振荡培养条件下的纤维素产量。在茶培养基中，用绿茶获得的纤维素产量最高（约0.20 g/L），高于红茶（约0.14 g/L）。HS培养基中的产量更高（约0.28 g/L），但静置培养和振荡培养之间没有差异。（1）H-NMR和（13）C-NMR光谱表明，该纤维素是纯的（不含乙酰基），且具有高结晶度。通过改变HS培养基中碳源和氮源的类型及水平，纤维素产量得到了提高。20 g/L的甘露醇与40 g/L的玉米浆配合使用时，可获得约2.64 g/L的高产。在茶培养基中，3 g/L的茶叶用量可获得最高的纤维素产量，向HS培养基中添加3 g/L的茶叶可使纤维素产量提高到3.34 g/L。总之，来自康普茶的木醋杆菌菌株具有与先前从水果中分离出的菌株不同的纤维素生产特性。所产纤维素为纯形式，具有很高的潜在应用价值。我们的研究首次对来自康普茶的木醋杆菌菌株的纤维素生产进行了广泛表征，表明其与不同来源菌株既有相似之处也有差异。
